Journal: Communications Biology
Article Title: Deletion of the diabetes candidate gene Slc16a13 in mice attenuates diet-induced ectopic lipid accumulation and insulin resistance
doi: 10.1038/s42003-021-02279-8
Figure Lengend Snippet: a Immunofluorescence co-staining of the plasma membrane marker wheat germ agglutinin and FLAG-tagged Slc16a13 in HEK- Slc16a13-FLAG cells. One representative staining is shown. b l -lactate uptake into HEK-control and HEK- Slc16a13 cells ( n = 3 for each concentration). Bars represent means ± SEM. ** p < 0.01, *** p < 0.001 determined using one-way ANOVA followed by Dunnett’s multiple comparison test. c Net l -lactate uptake as the difference between the uptake into HEK-control and HEK- Slc16a13 cells. Nonlinear regression curve and means ± SEM are displayed.
Article Snippet: K m value was assessed using nonlinear regression curve fit (GraphPad Prism 7).
Techniques: Immunofluorescence, Staining, Clinical Proteomics, Membrane, Marker, Control, Concentration Assay, Comparison